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cts n 2 supplement  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cts n 2 supplement
    Cts N 2 Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cts+n+2+supplement/Casein/pmc12796728-36-0-4
    Average 96 stars, based on 1 article reviews
    cts n 2 supplement - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Knock-Out:

    Article Title: cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness
    Article Snippet: .. On day 18, the 3D differentiation media was removed and switched to neural retina culture medium [CTS KnockOut DMEM/F12 (Gibco/Thermo Fisher Scientific; Cat#: A1370801), 2 mM GlutaMAX supplement (Gibco/Thermo Fisher Scientific; Cat#: 35050061), and CTS N-2 supplement (Cell Therapy Systems, Thermo Fisher Scientific, Grand Island, NY; Cat#: A1370701)]. .. On days 30–40, 10 mM DAPT (EMD Millipore; Cat#: 565770) was added to enhance photoreceptor cell production.

    Article Title: cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness.
    Article Snippet: .. On day 18, the 3D differentiation media was removed and switched to neural retina culture medium [CTS KnockOut DMEM/F12 (Gibco/Thermo Fisher Scientific; Cat#: A1370801), 2 mM GlutaMAX supplement (Gibco/Thermo Fisher Scientific; Cat#: 35050061), and CTS N-2 supplement (Cell Therapy Systems, Thermo Fisher Scientific, Grand Island, NY; Cat#: A1370701)]. .. On days 30–40, 10 mM DAPT (EMD Millipore; Cat#: 565770) was added to enhance photoreceptor cell production.

    Article Title: Generation of xenobiotic free retinofugal assembloids
    Article Snippet: ⁃ 0.80micron PES 25 mm Syringe Filter (JinTeng, Cat# PES-0.8-25) ⁃ 0.45micron PES Filter System (Dot Scientific, Cat#229702) ⁃ 0.22micron PES Filter System (Dot Scientific, Cat#229706) 100 mL CTS KnockOut Serum Replacement (Gibco, Cat# 12-618-013) 5 mL MEM non-essential amino acids (Gibco, Cat# 11-140-050) 5 mL CTS GlutaMAX (Gico, Cat# A1286001) 5 mL sodium pyruvate (Gibco, Cat# 11360-070) 200 μL β-mercaptoethanol (Sigma, Cat# M3148-25 ML) 1 mL Primocin o Supplement 50 mL aliquots with: o 10 μM ROCK inhibitor Y-27632 (Tocris, Cat# 1254) o 1.5 μM (55 ng/mL) BMP4 (R&D Systems) .. 489 mL CTS KnockOut DMEM/F12 (Gibco, Cat# A1370801) 5 mL CTS N-2 Supplement (Gibco, Cat# A1370701) 5 mL CTS GlutaMAX (Gibco) 1 mL Primocin ..

    Article Title: A protocol for the generation of xeno-free, cGMP-compliant patient-specific iPSCs from skin biopsy
    Article Snippet: .. Patient-specific iPSCs (from Protocol 2 above) BioSpherix Xvivo closed incubator/cell culture system (BioSpherix, Ltd.; Parish, NY) EVOS Digital microscope (Thermo Fisher Scientific; Waltham, MA; Cat#: AME 3300) Sorvall ST 8 Tabletop Centrifuge (Thermo Fisher Scientific; Waltham, MA; Cat#: 75007205) Scepter 2.0 Cell Counter and 40 μM sensors (EMD Millipore; Billerica, MA; Cat#s: PHCC20040 and PHCC40050) CTS TrypLE Select Enzyme (Gibco/Thermo Fisher Scientific, Grand Island, NY; Cat#: A1285901) Corning spheroid 96-well microplate, ultra-low attachment (Corning Life Sciences; Tewksbury, CA; Cat#: #4520 or CLS7007) ClipTip 30–300 μl multi-channel pipettor (Thermo Fisher Scientific; Grand Island, NY; Cat#: 4661140) 100 mm ultra-low attachment culture dishes (Corning Life Sciences; Tewksbury, CA; Cat#: 3262) 3D differentiation media 395 mL CTS KnockOut DMEM (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: A1286101) 50 mL heat-inactivated human serum (Innovative Research; Novi, MI; Cat#: IPLA-SERAB-HI) 100 mL CTS KnockOut Serum Replacement XenoFree medium (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: 12618013) 0.1 mM MEM non-essential amino acids (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: 11140-050) 1 mL Primocin (InvivoGen; San Diego, CA; Cat#: ant-pm-2) 1 mM Sodium Pyruvate (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: 11360-070) 0.1 mM 2-Mercaptoethanol (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: 21985-023) The following are added as supplements to the working aliquot of 3D Differentiation Media: 1% ECM (1% ECM mixture) human type 1 collagen (Advanced BioMatrix, Carlsbad, CA; Cat#: 5007-20ML) human type 3 collagen (Advanced BioMatrix, Carlsbad, CA; Cat#: 5021-10MG) human vitronectin (Advanced BioMatrix, Carlsbad, CA; Cat#: 5051-0.1MG) human fibronectin (Advanced BioMatrix, Carlsbad, CA; Cat#: 5050-1MG) 10 μM Y-27632 ROCK inhibitor (EMD Millipore; Billerica, MA; Cat#: 688000) 3 μM IWR1e (Cayman Chemical; Ann Arbor, MI; Cat#: 13659) 40 nM StemMACS CHIR99021 (Miltenyi Biotec Inc.; San Diego, CA; Cat#: 130-103-926) 100 nM SAG (Enzo Life Sciences; Farmingdale, NY, Cat#: ALX-270-426) Neural Retina culture medium (NR media) 489 mL CTS KnockOut DMEM/F12 (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: A1370801) 2 mM CTS GlutaMAX-I Supplement (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: A1286001) 1 mL Primocin (InvivoGen; San Diego, CA; Cat#: ant-pm-2) 5 mL CTS N-2 supplement (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: A1370701) The following is added to the working aliquot of NR media: 10 μM DAPT (EMD Millipore; Billerica, MA; Cat#: 565770). ..

    Microscopy:

    Article Title: A protocol for the generation of xeno-free, cGMP-compliant patient-specific iPSCs from skin biopsy
    Article Snippet: .. Patient-specific iPSCs (from Protocol 2 above) BioSpherix Xvivo closed incubator/cell culture system (BioSpherix, Ltd.; Parish, NY) EVOS Digital microscope (Thermo Fisher Scientific; Waltham, MA; Cat#: AME 3300) Sorvall ST 8 Tabletop Centrifuge (Thermo Fisher Scientific; Waltham, MA; Cat#: 75007205) Scepter 2.0 Cell Counter and 40 μM sensors (EMD Millipore; Billerica, MA; Cat#s: PHCC20040 and PHCC40050) CTS TrypLE Select Enzyme (Gibco/Thermo Fisher Scientific, Grand Island, NY; Cat#: A1285901) Corning spheroid 96-well microplate, ultra-low attachment (Corning Life Sciences; Tewksbury, CA; Cat#: #4520 or CLS7007) ClipTip 30–300 μl multi-channel pipettor (Thermo Fisher Scientific; Grand Island, NY; Cat#: 4661140) 100 mm ultra-low attachment culture dishes (Corning Life Sciences; Tewksbury, CA; Cat#: 3262) 3D differentiation media 395 mL CTS KnockOut DMEM (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: A1286101) 50 mL heat-inactivated human serum (Innovative Research; Novi, MI; Cat#: IPLA-SERAB-HI) 100 mL CTS KnockOut Serum Replacement XenoFree medium (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: 12618013) 0.1 mM MEM non-essential amino acids (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: 11140-050) 1 mL Primocin (InvivoGen; San Diego, CA; Cat#: ant-pm-2) 1 mM Sodium Pyruvate (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: 11360-070) 0.1 mM 2-Mercaptoethanol (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: 21985-023) The following are added as supplements to the working aliquot of 3D Differentiation Media: 1% ECM (1% ECM mixture) human type 1 collagen (Advanced BioMatrix, Carlsbad, CA; Cat#: 5007-20ML) human type 3 collagen (Advanced BioMatrix, Carlsbad, CA; Cat#: 5021-10MG) human vitronectin (Advanced BioMatrix, Carlsbad, CA; Cat#: 5051-0.1MG) human fibronectin (Advanced BioMatrix, Carlsbad, CA; Cat#: 5050-1MG) 10 μM Y-27632 ROCK inhibitor (EMD Millipore; Billerica, MA; Cat#: 688000) 3 μM IWR1e (Cayman Chemical; Ann Arbor, MI; Cat#: 13659) 40 nM StemMACS CHIR99021 (Miltenyi Biotec Inc.; San Diego, CA; Cat#: 130-103-926) 100 nM SAG (Enzo Life Sciences; Farmingdale, NY, Cat#: ALX-270-426) Neural Retina culture medium (NR media) 489 mL CTS KnockOut DMEM/F12 (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: A1370801) 2 mM CTS GlutaMAX-I Supplement (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: A1286001) 1 mL Primocin (InvivoGen; San Diego, CA; Cat#: ant-pm-2) 5 mL CTS N-2 supplement (Gibco/Thermo Fisher Scientific; Grand Island, NY; Cat#: A1370701) The following is added to the working aliquot of NR media: 10 μM DAPT (EMD Millipore; Billerica, MA; Cat#: 565770). ..

    other:

    Article Title: Safety and tolerability of RPESC-RPE transplantation in patients with dry age-related macular degeneration: Low-dose clinical outcomes
    Article Snippet: CTS N-2 Supplement , Thermo Fisher Scientific , A1370701.

    Article Title: Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
    Article Snippet: CTS N-2 Supplement , Life Technologies , A13707-01.



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